What is methylation array?

Methylation arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

What is a BeadChip array?

Bead-Based Microarray Technology Illumina microarray technology (also known as BeadArray technology) uses silica microbeads. On the surface of each array, or BeadChip, hundreds of thousands to millions of genotypes for a single individual can be assayed at once.

What is CpG in DNA?

Definition. CpG is shorthand for 5’—C—phosphate—G—3′ , that is, cytosine and guanine separated by only one phosphate group; phosphate links any two nucleosides together in DNA. The CpG notation is therefore to be interpreted as the cytosine being 5 prime to the guanine base.

How many CPGS are in the human genome?

30,000 CpG
The human genome contains ∼30,000 CpG islands (CGIs), long stretches (0.5–2 kb) of DNA with unusually elevated levels of CpG dinucleotides. Many occur at genes’ promoters, and their DNA nearly always remains unmethylated.

Is GenomeStudio free?

Access to GenomeStudio 2.0 is now free.

What is a genotyping array?

Genotyping arrays, often called SNP (single nucleotide polymorphism) arrays, provide information on hundreds of thousands of DNA variants at a reasonable cost. By running all your customers’ samples on an array, you’ll have data for hundreds of thousands of DNA variants.

Why is it called CPG?

CpG is shorthand for 5’—C—phosphate—G—3′ , that is, cytosine and guanine separated by only one phosphate group; phosphate links any two nucleosides together in DNA.

Why is methylation of DNA important?

DNA methylation is essential for silencing retroviral elements, regulating tissue-specific gene expression, genomic imprinting, and X chromosome inactivation. Importantly, DNA methylation in different genomic regions may exert different influences on gene activities based on the underlying genetic sequence.

What is the content descriptor for the humanmethylation450k chip?

Content descriptor file for the HumanMethylation450K chip, which is required for Accession Methylation BeadChips into LIMS. A list of SNPs that could potentially impact methylation array results if present in the test population.

How many methylation sites are on the Infinium beadchip?

The Infinium HumanMethylation450 BeadChip features more than 450,000 methylation sites, within and outside of CpG islands.

What to use as a sample sheet for infinium hd methylation assay?

A comma-separated values file to use as a sample sheet template for the Infinium HD Methylation assay. Product Information Sheet, Data Sheet, and product files for the HumanMethylation450 BeadChip. Content descriptor file for the HumanMethylation450K chip, which is required for Accession Methylation BeadChips into LIMS.

How many methylation markers are there in Illumina?

Illumina Methylation Arrays Our Infinium MethylationEPIC and Mouse Methylation BeadChips allow you to interrogate 850,000 or 285,000 markers, respectively, across the genome at single-nucleotide resolution.