What are the limitations of double reciprocal plot?
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What are the limitations of double reciprocal plot?
Because they involve grossly uneven weightings of points, the linear and formally similar double reciprocal Benesi-Hildebrand and Lineweaver-Burke plots should never be used to resolve equilibrium and enzyme kinetic results. In such plots weightings from one end to the other may vary as much as a thousand or more.
What is a double reciprocal plot?
The double-reciprocal (also known as the Lineweaver-Burk) plot is created by plotting the inverse initial velocity (1/V0) as a function of the inverse of the substrate concentration (1/[S]). The Vmax can be accurately determined and thus KM can also be determined with accuracy because a straight line is formed.
What is the importance of double reciprocal plot?
The double-reciprocal, or Lineweaver-Burke plot appears in virtually every treatment of enzyme kinetics and enzyme inhibition from the textbook level to research papers and monographs. It is also widely used in research to extract kinetic parameters, such as the Vmax, Km, and Ki values.
Why are Lineweaver-Burk plots problematic?
Problems with Lineweaver-Burk The y-axis of the plot takes the reciprocal of the rate of reaction, meaning small errors in measurement are more noticeable. Additionally because most points on the plot are found far to the right of the y-axis.
Why is Michaelis Menten plot better than Lineweaver-Burk?
For instance; Lineweaver-Burke plot, the most favoured plot by researchers, has two distinct advantages over the Michaelis-Menten plot, in that it gives a more accurate estimate of Vmax and more accurate information about inhibition. It increases the precision by linearizing the data.
Which of the following is the slope of double-reciprocal plot?
Figure 8.36. A Double-Reciprocal or Lineweaver-Burk Plot. A double-reciprocal plot of enzyme kinetics is generated by plotting 1/V0 as a function 1/[S]. The slope is the KM/Vmax, the intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis (more…)
What are the advantages of the Lineweaver-Burk plot?
What is the purpose of a Lineweaver-Burk plot?
Uses of Lineweaver–Burk Plot Used to determine important terms in enzyme kinetics, such as Kmand Vmax, before the wide availability of powerful computers and non-linear regression software. Gives a quick, visual impression of the different forms of enzyme inhibition.