Miscellaneous

Does DMEM have pyruvate?

Contents

Does DMEM have pyruvate?

DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

What is DMEM low glucose?

How much glucose is in high glucose DMEM?

Specifications

Glucose Concentration: 4500 mg/L
Volume (Metric): 500 ml
Product Line: Gibco™
Form: Liquid
Glutamine: L-Glutamine

What is DMEM made of?

DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.

Does RPMI need pyruvate?

RPMI, unlike DMEM, does not contain sodium pyruvate.

Is sodium pyruvate the same as pyruvate?

Sodium pyruvate is a salt of the conjugate anion form of pyruvic acid, known as pyruvate.

How much glucose in DMEM is low?

DMEM, Low Glucose Contains 1000 mg\L D-glucose, 110mg/L Sodium Pyruvate. This preparation is without L-Glutamine.

Does DMEM contain glucose?

Can DMEM substitute EMEM?

DMEM is a liquid serum-free media and a variant of BME, or Basal Medium Eagle, modified to contain up to four times more vitamins and amino acids, and two to four times more glucose than EMEM; making it an ideal supplement for most cell culture media applications.

Does DMEM have insulin?

In low-serum DMEM containing 2.5 micrograms/ml insulin there was a dose-dependent increase in cell growth with EGF and TFG alpha at concentrations of 0.5-10.0 nM.

How do I get 10% FBS DMEM?

(When you make 10% FBS, 1% Pen/Strep-containing DMEM, you just directly add 50 ml of FBS and 5 ml of Pen/Strep.)

What kind of pyruvate is in DMEM?

DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use. Gibco® DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK.

Can you use DMEM with high glucose levels?

DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

What makes DMEM unique from other essential media?

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Are there any lipids or proteins in DMEM?

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO 2 environment to maintain physiological pH. For Research Use Only.